Microarray Measures of Gene Expression In Support of Oligonucleotide Gene Based Immunomodulator Discovery

Can one prolong graft survival in transplant patients by using novel therapeutics oligonucleotides to decrease the ability of the graft to trigger the host immune system. Such a strategy is in contrast to the more common approach involving host immunosuppression. We are investigating a series of oligonucleotides that were designed to downregulate the expression of MHC class I and class II genes. In particular, we are interested in the extent to which constitutive and induced expression of MHC genes can be reduced by therapeutic oligonucleotides, and the resultant effect on the host immune response. In Project One, the Hunt Group is implementing strategies to acquire, evaluate, and use microarray measured gene expression to facilitate the above approach.

Collaborators: Richard Shafer.


The premise of the agent paradigm, its related theory and methodologies together with advances in multilevel modeling of complex systems of interactions opened new frontiers for advancing the physical, natural, social, military, and information sciences and engineering...